Dr. Alexandru Movila
Dr. Alexandru Movila’s research goal is to establish the impact of ceramides produced by the key periodontal pathogen Porphyromonas gingivalis on MIF-mediate homing of Osteoclast Precursors (OCPs) to periodontal lesions in the context of age-dependent bone loss.
His training being a T32-NIDCR Fellow in the field of bone immunology and osteolysis lesion development at the Forsyth Institute known for pioneering biomedical research and transformational healthcare practices. He has studied the locomotion of macrophages, OCPs and osteoclast formation in response to various inflammatory stimuli using mouse models of periodontitis and calvaria osteolytic lesions as well as in a single-cell droplet microfluidic chip. He has also optimized a ligature-induced mouse model of periodontitis to sample gingival cevicular fluid. He has had extensive training in protocols required to carry out the proposed study, namely FACS-sorting of OCPs, in vitro RANKL-induced osteoclastogenesis, and mAbs design as well as in sample preparation and data analysis using microcomputer tomography.
Dr. Movila has established in vitro as well as in vivo protocols to evaluate the impact of macrophage migration inhibitory factor (MIF) on periodontitis. His research group is active in:
Studying age-dependent effects between MARCO and TLR4 on particle phagocytosis by macrophages,
Developing in vivo imaging protocols using the cutting-edge Cellvizio,
Brucker’s technologies to study periodontal disease pathogenesis in mice, and
Establishing the protective role of acid ceramidase against gingvalis-induced inflammation.